Human CD19 Protein (CD1-HM119)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human CD19, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Pro20-Lys291

Catalog: CD1-HM119

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Product Description	Recombinant Human CD19 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Pro20-Lys291.
Product Background	CD19 is a 95 kDa transmembrane glycoprotein that plays a central role in B cell activation and humoral immune responses.Functions as coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes. Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens. Activates signaling pathways that lead to the activation of phosphatidylinositol 3-kinase and the mobilization of intracellular Ca2 stores.
Product Category	Recombinant Protein

Target	CD19
Synonyms	CD19; B4; CVID3; Leu-12; MGC12802
Gene/Protein ID	P15391-1
Species	Human
Exact Sequence	Pro20-Lys291
Molecular Weight	The protein has a predicted MW of 34.7 kDa. Due to glycosylation, the protein migrates to 50-60 KDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD19, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD19, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Coltuximab, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).
	Immobilized Human CD19, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Inebilizumab, hFc Tag with the EC50 of 11.5ng/ml determined by ELISA.
	Immobilized Human CD19, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for FMC63, mFc Tag with the EC50 of 10.0ng/ml determined by ELISA (QC Test).
	Immobilized Human CD19, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Denintuzumab, hFc Tag with the EC50 of 2.1ng/ml determined by ELISA.
	Human CD19 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.