Human CD27 Ligand/CD70 Protein (CD7-HM270)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln39-Pro193

Catalog: CD7-HM270

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Product Description	Recombinant Human CD27 Ligand/CD70 Protein is expressed from HEK293 with hFc at the N-Terminus.It contains Gln39-Pro193.
Product Background	CD70, also named CD27 ligand (CD27L), is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7.- CD70 is a cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Product Category	Recombinant Protein

Target	CD27 Ligand/CD70
Synonyms	CD70 molecule; CD70; TNFSF7; CD27 Ligand; CD27-L; CD27LG;-Ki-24 antigen; TNFSF7G
Gene/Protein ID	P32970-1
Species	Human
Exact Sequence	Gln39-Pro193
Molecular Weight	The protein has a predicted MW of 42.7 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD27, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
	The purity of Human CD27 Ligand is greater than 95% as determined by SEC-HPLC.
	Human CD27 Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.