Human CD28H/IGPR-1 Protein (CD8-HM28H)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-H7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.89ug/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu23-Gly150

Catalog: CD8-HM28H

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Product Description	Recombinant Human CD28H/IGPR-1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu23-Gly150.
Product Background	CD28H is constitutively expressed on all naive T cells. Repetitive antigenic exposure, however, induces a complete loss of CD28H on many T cells, and CD28H negative T cells have a phenotype of terminal differentiation and senescence. After extensive screening in a receptor array, a B7-like molecule, B7 homologue 5 (B7-H5), was identified as a specific ligand for CD28H.
Product Category	Recombinant Protein

Target	CD28H/IGPR-1
Synonyms	TMIGD2; IGPR1; IGPR-1; CD28H; TPC1
Gene/Protein ID	Q96BF3-1
Species	Human
Exact Sequence	Leu23-Gly150
Molecular Weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human B7-H7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.89ug/ml determined by ELISA (QC Test). The affinity constant of 7.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Cynomolgus B7-H7, His Tag captured on CM5 Chip via anti-his antibody can bind Human CD28H, hFc Tag with an affinity constant of 7.86 nM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-H7, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human CD28H, hFc Tag with the EC50 of 0.89ug/ml determined by ELISA (QC Test).
	The purity of Human CD28H is greater than 95% as determined by SEC-HPLC.
	Human CD28H on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.