Human CD48/SLAMF2 Protein (SLA-HM148)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln27-Ser220

Catalog: SLA-HM148

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Product Description	Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln27-Ser220.
Product Background	CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.
Product Category	Recombinant Protein

Target	CD48/SLAMF2
Synonyms	CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;- mCD48; MEM-102; TCT.1
Gene/Protein ID	P09326-1
Species	Human
Exact Sequence	Gln27-Ser220
Molecular Weight	The protein has a predicted MW of 23.4 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD48, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human 2B4, hFc Tag with the EC50 of 89.8ng/ml determined by ELISA.
	The purity of Human CD48 is greater than 95% as determined by SEC-HPLC.
	Human CD48 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.