Human CD5 Protein (CD5-HM501)

Express System: HEK293
Product Tag: C-hFc-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD5 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 14.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Arg25-Asn371

Catalog: CD5-HM501

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Product Description	Recombinant Human CD5 Protein is expressed from HEK293 with hFc tag and Avi tag at the C-Terminus.It contains Arg25-Asn371.
Product Background	CD5: a type I transmembrane protein found on T cells, thymocytes, and some B cells that is a ligand for CD72 and is involved in cellular activation or adhesion; expressed in B-cell chronic lymphocytic leukemia and T-cell lymphoma.
Product Category	Recombinant Protein

Target	CD5
Synonyms	CD5 molecule; CD5; LEU1; T1; CD5 antigen
Gene/Protein ID	P06127
Species	Human
Exact Sequence	Arg25-Asn371
Molecular Weight	The protein has a predicted MW of 67.1 kDa. Due to glycosylation, the protein migrates to 75-82 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD5 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 14.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD5 at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD5 Antibody, hFc Tag with the EC50 14.6ng/ml determined by ELISA.
	The purity of Human CD5 is greater than 95% as determined by SEC-HPLC.
	Human CD5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.