Human CD55 Protein (CD5-HM105)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD55, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp35-Ser353

Catalog: CD5-HM105

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Product Description	Recombinant Human CD55 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp35-Ser353.
Product Background	Decay Accelerating Factor (or CD55) is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection.
Product Category	Recombinant Protein

Target	CD55
Synonyms	DAF-GPI; CD55; Cd55a; Daf; Daf1; DAF; CR; CROM; TC
Gene/Protein ID	P08174-1
Species	Human
Exact Sequence	Asp35-Ser353
Molecular Weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 70-75 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD55, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD55, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD55 Antibody, hFc Tag with the EC50 of 14.3ng/ml determined by ELISA.
	The purity of Human CD55 is greater than 95% as determined by SEC-HPLC.
	Human CD55 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.