Human CD6 Protein (CD6-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: His18-Leu402

Catalog: CD6-HM101

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Product Description	Recombinant Human CD6 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains His18-Leu402.
Product Background	The cell surface glycoprotein CD6 is expressed on leukocytes and mediates T cell trafficking across endothelial cell barriers. CD6 is a lymphocyte surface co-receptor physically associated with the T-cell receptor (TCR)/CD3 complex at the center of the immunological synapse.
Product Category	Recombinant Protein

Target	CD6
Gene/Protein ID	NP_006716.3
Species	Human
Exact Sequence	His18-Leu402
Molecular Weight	The protein has a predicted MW of 42.14 kDa. Due to glycosylation, the protein migrates to 70-90 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.53 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human ALCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD6, His Tag with an affinity constant of 0.53 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CD6, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human ALCAM, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
	The purity of Human CD6 is greater than 95% as determined by SEC-HPLC.
	Human CD6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.