Human CEACAM-1/CD66a Protein (CEA-HM201)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln35-Gly428

Catalog: CEA-HM201

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Product Description	Recombinant Human CEACAM-1/CD66a Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln35-Gly428.
Product Background	Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM-1) is the major antigen of the CD66 cluster of granulocyte differentiation antigens. The present study aimed to assess the biological function of CEACAM-1 on the growth of human colorectal cancer (CRC) cells in vitro.
Product Category	Recombinant Protein

Target	CEACAM-1/CD66a
Synonyms	BGP; BGP1; BGPI; CEACAM1; CD66a
Gene/Protein ID	P13688-1
Species	Human
Exact Sequence	Gln35-Gly428
Molecular Weight	The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 68-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test). The affinity constant of 0.37 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CEACAM-1, His Tag immobilized on CM5 Chip can bind Human CEACAM-7, His Tag with an affinity constant of 0.37 uM as determined in SPR assay (Biacore T200).
	Immobilized Human CEACAM-1 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM-1 Antibody, hFc Tag with the EC50 of 3.2ng/ml determined by ELISA (QC Test).
	The purity of Human CEACAM-1 is greater than 95% as determined by SEC-HPLC.
	Human CEACAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.