Human DLL3 Protein (DLL-HM103)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human DLL3, His Tag at 0.1ug/mL (100ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala27-Arg490

Catalog: DLL-HM103

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Product Description	Recombinant Human DLL3 is expressed from HEK293 with His tag at the N-Terminus.It contains Ala27-Arg490.
Product Background	Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Product Category	Recombinant Protein

Target	DLL3
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Gene/Protein ID	Q9NYJ7-1
Species	Human
Exact Sequence	Ala27-Arg490
Molecular Weight	The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human DLL3, His Tag at 0.1ug/mL (100ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA (QC Test). The affinity constant of 0.22 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS, 200mM Arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-DLL3 Antibody captured on CM5 Chip via Protein A can bind Human DLL3, His Tag with an affinity constant of 0.22 nM as determined in SPR assay (Biacore T200).
	Immobilized Human DLL3, His Tag at 0.1ug/mL (100ul/well). Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 4.5ng/ml determined by ELISA (QC Test).
	Human DLL3 on Tris-Bis PAGE under reduced- condition. The purity is greater than 95%.