Human DPPIV/CD26 Protein (DPV-HM126)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).. See testing image for detail.
Exact Sequence: Asn29-Pro766

Catalog: DPV-HM126

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Product Description	Recombinant Human DPPIV/CD26 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn29-Pro766.
Product Background	CD26/dipeptidyl peptidase (DPP)IV is a membrane-bound protein found in many cell types of the body, and a soluble form is present in body fluids. There is longstanding evidence that various primary tumors and also metastases express DPPIV/CD26 to a variable extent.
Product Category	Recombinant Protein

Target	DPPIV/CD26
Synonyms	ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5
Gene/Protein ID	P27487
Species	Human
Exact Sequence	Asn29-Pro766
Molecular Weight	The protein has a predicted MW of 86.39 kDa. Due to glycosylation, the protein migrates to 90-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test).. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human DPPIV, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-DPPIV Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA (QC Test)..
	Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >8500 pmol/min/µg (QC Test).
	The purity of Human DPPIV is greater than 95% as determined by SEC-HPLC.
	Human DPPIV on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.