Human Endoglin/CD105 Protein (EDG-HM401)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Endoglin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu26-Gly586

Catalog: EDG-HM401

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Product Description	Recombinant Human Endoglin/CD105 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu26-Gly586.
Product Background	Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is a vascular endothelium glycoprotein that plays an important role in the regulation of angiogenesis. Required for normal structure and integrity of adult vasculature (PubMed:7894484). Regulates the migration of vascular endothelial cells. Required for normal extraembryonic angiogenesis and for embryonic heart development. May regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis.
Product Category	Recombinant Protein

Target	Endoglin/CD105
Synonyms	CD105; Endoglin; ENG; ORW; ORW1; END; HHT1
Gene/Protein ID	P17813-1
Species	Human
Exact Sequence	Glu26-Gly586
Molecular Weight	The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Endoglin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Endoglin, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
	The purity of Human Endoglin is greater than 95% as determined by SEC-HPLC.
	Human Endoglin on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.