Human EphA7 Protein (EPH-HM1A7)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln28-Val555

Catalog: EPH-HM1A7

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Product Description	Recombinant Human EphA7 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln28-Val555.
Product Background	Ephrin receptor A7 (EphA7) is a member of the Eph receptor family. It is widely involved in signal transduction between cells, regulates cell proliferation and differentiation, and participates in developing neural tubes and brain. In addition, EphA7 also has a dual role of tumor promoter and tumor suppressor.
Product Category	Recombinant Protein

Target	EphA7
Gene/Protein ID	NP_004431.1
Species	Human
Exact Sequence	Gln28-Val555
Molecular Weight	The protein has a predicted MW of 60.13 kDa. Due to glycosylation, the protein migrates to 65-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.113 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human EphA7, His Tag immobilized on CM5 Chip can bind Human Ephrin-A4, His Tag with an affinity constant of 0.113 uM as determined in SPR assay (Biacore T200).
	Immobilized Human EphA7, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human Ephrin-A4, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA (QC Test).
	The purity of Human EphA7 is greater than 95% as determined by SEC-HPLC.
	Human EphA7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.