Human Fas/TNFRSF6/CD95 Protein (FAS-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln26-Asn173

Catalog: FAS-HM101

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Product Description	Recombinant Human Fas/TNFRSF6/CD95 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln26-Asn173.
Product Background	CD95 (also known as Fas) is a member of the tumor necrosis factor receptor (TNFR) superfamily. Its cognate ligand, CD95L, is implicated in immune homeostasis and immune surveillance. Mutations in this receptor are associated with a loss of apoptotic signaling and have been detected in an autoimmune disorder called autoimmune lymphoproliferative syndrome (ALPS) type Ia, which shares some clinical features with systemic lupus erythematosus (SLE).
Product Category	Recombinant Protein

Target	Fas/TNFRSF6/CD95
Synonyms	FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR
Gene/Protein ID	P25445-1
Species	Human
Exact Sequence	Gln26-Asn173
Molecular Weight	The protein has a predicted MW of 13.7 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 19.45 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human Fas, His Tag immobilized on CM5 Chip can bind Human Fas Ligand, His Tag with an affinity constant of 19.45 nM as determined in SPR assay (Biacore T200).
	Human Fas on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.