Human FcRn Protein (FRN-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human FcRn, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

Catalog: FRN-HM101

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Product Description	Recombinant Human FcRn Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ala24-Ser297(FCGRT) & Ile21-Met119(B2M).
Product Background	The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Product Category	Recombinant Protein

Target	FcRn
Synonyms	FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
Gene/Protein ID	P55899-1(FCGRT)&P61769-1(B2M)
Species	Human
Exact Sequence	Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)
Molecular Weight	The protein has a predicted MW of 31.5 kDa (FCGRT) & 11.7 kDa (B2M). Due to glycosylation, the protein migrates to 35-40 kDa (FCGRT) & 13-14 kDa (B2M) based on Tris-Bis PAGE result.
Activity	Immobilized Human FcRn, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.28 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Human IgG1 Fc, No Tag with an affinity constant of 0.28 uM as determined in SPR assay (Biacore T200).
	Immobilized Human FcRn, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-FcRn Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
	The purity of Human FcRn is greater than 95% as determined by SEC-HPLC.
	Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.