Human FGF-7/KGF Protein (KGF-HE101)

Express System: E.coli
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys32-Thr194

Catalog: KGF-HE101

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Product Description	Recombinant Human FGF-7/KGF Protein is expressed from E.coli with His tag at the N-Terminus.It contains Cys32-Thr194.
Product Background	The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
Product Category	Recombinant Protein

Target	FGF-7/KGF
Synonyms	FGF-7; HBGF-7; KGF
Gene/Protein ID	P21781-1
Species	Human
Exact Sequence	Cys32-Thr194
Molecular Weight	The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.
Activity	The affinity constant of 18.91 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 50mM Tris, 250mM NaCl (pH 7.5).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human FGF-7, His Tag immobilized on CM5 Chip can bind Human FGFR2 beta (IIIb) , His Tag with an affinity constant of 18.91 nM as determined in SPR assay (Biacore T200).
	The purity of Human FGF-7 is greater than 95% as determined by SEC-HPLC.
	Human FGF-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.