Human FGF8a-Protein (FGF-HE08A)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Gln23-Arg204

Catalog: FGF-HE08A

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Product Description Recombinant Human FGF8a-Protein is expressed from E.coli without tag.It contains Gln23-Arg204.

Product Background

 Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.

Product Category

 Recombinant Protein

Target

 FGF8a

Gene/Protein ID

 P55075-2

Species

 Human

Exact Sequence

 Gln23-Arg204
Molecular Weight The protein has a predicted MW of 21.20 kDa same as Tris-Bis PAGE result.

Product Tag

 No Tag

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 20mM Tris-HCl, 300mM NaCl (pH 8.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 0.5 - 2 ug/mL.
Human FGF8a-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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