Human FGF8b-Protein (FGF-HE08B)

  • Express System: E.coli
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Exact Sequence: Gln23-Arg215

Catalog: FGF-HE08B

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Product Description Recombinant Human FGF8b-Protein is expressed from E.coli without tag.It contains Gln23-Arg215.

Product Background

 Two of the four human FGF8 splice isoforms, FGF8a and FGF8b, are expressed in the mid-hindbrain region during development. Although the only difference between these isoforms is the presence of an additional 11 amino acids at the N terminus of FGF8b, these isoforms possess remarkably different abilities to pattern the midbrain and anterior hindbrain.

Product Category

 Recombinant Protein

Target

 FGF8b

Gene/Protein ID

 P55075-3

Species

 Human

Exact Sequence

 Gln23-Arg215
Molecular Weight The protein has a predicted MW of 22.39 kDa same as Tris-Bis PAGE result.

Product Tag

 No Tag

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 20mM Tris, 300mM NaCl (pH 8.5). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblasts. The ED50-for this effect is 5-20 ng/mL.
Human FGF8b-on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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