Human Galectin 1 Protein (GAL-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Galectin 1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Galectin 1 Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala2-Asp135

Catalog: GAL-HM201

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Product Description	Recombinant Human Galectin 1 Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala2-Asp135.
Product Background	Galectin 1(Gal-1), a β-galactoside binding mammalian lectin of 14KDa, is implicated in many signalling pathways, immune responses associated with cancer progression and immune disorders. Inhibition of human Gal-1 has been regarded as one of the potential therapeutic approaches for the treatment of cancer, as it plays a major role in tumour development and metastasis by modulating various biological functions viz. apoptosis, angiogenesis, migration, cell immune escape.
Product Category	Recombinant Protein

Target	GAL1
Synonyms	Galectin-1; HLBP14; S-Lac lectin 1; LGALS1; GAL1; GBP; DKFZp686E23103
Gene/Protein ID	P09382
Species	Human
Exact Sequence	Ala2-Asp135
Molecular Weight	The protein has a predicted MW of 41.9 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Galectin 1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Galectin 1 Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Galectin 1, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-Galectin 1 Antibody, hFc Tag with the EC50 of 41.1ng/ml determined by ELISA.
	The purity of Human Galectin 1 is greater than 95% as determined by SEC-HPLC.
	Human Galectin 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.