Human GIP Protein (GIP-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GIP, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu22-Gln93

Catalog: GIP-HM101

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Product Description	Recombinant Human GIP Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu22-Gln93.
Product Background	The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals.
Product Category	Recombinant Protein

Target	GIP
Synonyms	GIP; Incretin
Gene/Protein ID	P09681
Species	Human
Exact Sequence	Glu22-Gln93
Molecular Weight	The protein has a predicted MW of 9.2 kDa. Due to glycosylation, the protein migrates to 15-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GIP, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GIP, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GIP Antibody, hFc Tag with the EC50 of 17.3ng/ml determined by ELISA.
	The purity of Human GIP is greater than 95% as determined by SEC-HPLC.
	Human GIP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.