Human GPVI Protein (GP6-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GPVI, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gln21-Lys267

Catalog: GP6-HM101

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Product Description	Recombinant Human GPVI Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln21-Lys267.
Product Background	Although platelets are best known for their role in hemostasis, they are also crucial in development, host defense, inflammation, and tissue repair. Many of these roles are regulated by the immune-like receptors glycoprotein VI (GPVI) and C-type lectin receptor 2 (CLEC-2), which signal through an immunoreceptor tyrosine-based activation motif (ITAM). GPVI is activated by collagen in the subendothelial matrix, by fibrin and fibrinogen in the thrombus, and by a remarkable number of other ligands.
Product Category	Recombinant Protein

Target	GPVI
Synonyms	GPVI; Gp6; Glycoprotein 6; MGC138168
Gene/Protein ID	Q9HCN6-1
Species	Human
Exact Sequence	Gln21-Lys267
Molecular Weight	The protein has a predicted MW of 28 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human GPVI, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human GPVI, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-GPVI Antibody, hFc Tag with the EC50 of 4.2ng/ml determined by ELISA.
	The purity of Human GPVI is greater than 95% as determined by SEC-HPLC.
	Human GPVI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.