Human HRG/HPRG-Protein (HRG-HM101)
Catalog: HRG-HM101
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| Product Description | Recombinant Human HRG/HPRG-Protein is expressed from HEK293 with His tag at the C-terminus.It contains Val19-Lys525. |
Product Background |
Histidine-rich glycoprotein (HRG) is a 75 kDa glycoprotein synthesized in the liver whose plasma concentration is 100-150 ug/ml. HRG has been shown to modulate sepsis-related biological reactions by binding to several substances and cells, including heparin, factor XII, fibrinogen, thrombospondin, plasminogen, C1q, IgG, heme, LPS, dead cells, bacteria, and fungi. |
Product Category |
Recombinant Protein |
Target |
HRG/HPRG |
Gene/Protein ID |
NP_000403.1 |
Species |
Human |
Exact Sequence |
Val19-Lys525 |
| Molecular Weight | The protein has a predicted MW of 58.75 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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The purity of Human HRG is greater than 95% as determined by SEC-HPLC. |
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Human HRG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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