Human HVEM/TNFRSF14 Protein (HVE-HM211)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu39-Val202

Catalog: HVE-HM211

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Product Description	Recombinant Human HVEM/TNFRSF14 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu39-Val202.
Product Background	Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor of the TNF-receptor superfamily.- Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling.
Product Category	Recombinant Protein

Target	HVEM/TNFRSF14
Synonyms	CD270; CD40-like protein;- HVEM; LIGHTR; ATAR; TNFRSF14; TR2
Gene/Protein ID	Q92956-1
Species	Human
Exact Sequence	Leu39-Val202
Molecular Weight	The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA. The affinity constant of 1.44 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human HVEM, hFc Tag captured on CM5 Chip via Protein A can bind Human CD160, His Tag with an affinity constant of 1.44 uM as determined in SPR assay (Biacore T200).
	Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA.
	Immobilized Human BTLA, His Tag at 1ug/ml (100ul/Well). Dose response curve for Human HVEM, hFc Tag with the EC50 of 42.5ng/ml determined by ELISA (QC Test).
	Human HVEM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.