Human IFN alpha 1 Protein (IFN-HM2A1)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Cys24-Glu189

Catalog: IFN-HM2A1

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Product Description	Recombinant Human IFN alpha 1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Cys24-Glu189.
Product Background	IFN-α, a cytokine expressed in human islets from individuals affected by type 1 diabetes, plays a key role in the pathogenesis of diabetes by upregulating inflammation, endoplasmic reticulum (ER) stress and MHC class I overexpression, three hallmarks of islet histology in early type 1 diabetes.
Product Category	Recombinant Protein

Target	IFN alpha 1
Synonyms	Interferon alpha-1/13;IFN-alpha-1/13;Interferon alpha-D;LeIF D; IFNalpha 1; IFN alpha1;IFNA1;IFN-alpha-1;Interferon alpha 1
Gene/Protein ID	P01562
Species	Human
Exact Sequence	Cys24-Glu189
Molecular Weight	The protein has a predicted MW of 46.14 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
Activity	The affinity constant of 0.12 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IFN alpha/beta R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 0.12 uM as determined in SPR assay (Biacore T200).
	Human IFN alpha/beta R2, His Tag captured on CM5 Chip via anti-his antibody can bind Human IFN alpha 1, hFc Tag with an affinity constant of 11.90 nM as determined in SPR assay (Biacore T200) (QC Test).
	The purity of Human IFN alpha 1 is greater than 95% as determined by SEC-HPLC.
	Human IFN alpha 1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.