Human IGFI Protein (IGF-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly49-Ala118

Catalog: IGF-HM201

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Product Description	Recombinant Human IGFI Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Gly49-Ala118.
Product Background	The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins.Insulin-like growth factor I (IGF-I) is a polypeptide hormone produced mainly by the liver in response to the endocrine GH stimulus, but it is also secreted by multiple tissues for autocrine/paracrine purposes.
Product Category	Recombinant Protein

Target	IGFI
Gene/Protein ID	P05019-1
Species	Human
Exact Sequence	Gly49-Ala118
Molecular Weight	The protein has a predicted MW of 33.18 kDa. Due to glycosylation, the protein migrates to 38-43 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA (QC Test). The affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IGF1R, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC50 of 1.25ug/ml determined by ELISA (QC Test).
	The purity of Human IGFI is greater than 95% as determined by SEC-HPLC.
	Human IGFI on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.