Human IGFII Protein (IGF-HM202)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala25-Glu91

Catalog: IGF-HM202

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Product Description	Recombinant Human IGFII Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala25-Glu91.
Product Background	The insulin family consists of insulin, insulin-like growth factor 1 (IGF-1), insulin-like growth factor 2 (IGF-2), their receptors (IR, IGF-1R and IGF-2R), and their binding proteins. All three ligands are involved in cell proliferation, apoptosis, protein synthesis and metabolism due to their homologous sequences and structural similarities. Insulin-like growth factor 2, a member of the insulin family, plays an important role in embryonic development, metabolic disorders, and tumorigenesis by combining with three receptors with different degrees of affinity.
Product Category	Recombinant Protein

Target	IGFII
Gene/Protein ID	P01344-1
Species	Human
Exact Sequence	Ala25-Glu91
Molecular Weight	The protein has a predicted MW of 33.00 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA. See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human IGFBP-3, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human IGF II, hFc Tag with the EC50 of 0.19ug/ml determined by ELISA.
	The purity of Human IGFII is greater than 95% as determined by SEC-HPLC.
	Human IGFII on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.