Human IL-1 alpha/IL-1A Protein (IL1-HE00A)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ser113-Ala271

Catalog: IL1-HE00A

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Product Description	Recombinant Human IL-1 alpha/IL-1A Protein is expressed from E.coli without tag.It contains Ser113-Ala271.
Product Background	The interleukin (IL)-1 family of cytokines is currently comprised of 11 members that have pleiotropic functions in inflammation and cancer. IL-1α and IL-1β were the first members of the IL-1 family to be described, and both signal via the same receptor, IL-1R. Over the last decade, much progress has been made in our understanding of biogenesis of IL-1β and its functions in human diseases.
Product Category	Recombinant Protein

Target	IL-1 alpha/IL1A
Synonyms	Interleukin-1 alpha; IL-1 alpha; Hematopoietin-1; IL1A; IL1F1; IL-1 ALPHA; IL1α; IL-1A; IL1
Gene/Protein ID	NP_000566.3
Species	Human
Exact Sequence	Ser113-Ala271
Molecular Weight	The protein has a predicted MW of 18 kDa same as Tris-Bis PAGE result.
Activity	The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
	The purity of Human IL-1 alpha is greater than 95% as determined by SEC-HPLC.
	Human IL-1 alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.