Human IL-1 Beta/IL-1F2 Protein (IL1-HE40B)

Express System: E.coli
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala117-Ser269

Catalog: IL1-HE40B

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Product Description	Recombinant Human IL-1 Beta/IL-1F2 Protein is expressed from E.coil with His tag and Avi tag at the C-Terminus.It contains Ala117-Ser269.
Product Background	Interleukin-1 beta (IL-1β) is induced by inflammatory signals in a broad number of immune cell types. IL-1β (and IL-18) are the only cytokines which are processed by caspase-1 after inflammasome-mediated activation. IL-1 signaling activates innate immune cells including antigen presenting cells, and drives polarization of CD4 T cells towards T helper type (Th) 1 and Th17 cells.
Product Category	Recombinant Protein

Target	IL-1 Beta/IL-1F2
Synonyms	Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B
Gene/Protein ID	P01584
Species	Human
Exact Sequence	Ala117-Ser269
Molecular Weight	The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 0.88 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1R2, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-1 Beta, His Tag with an affinity constant of 0.88 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-1 Beta, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-1R2, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	The purity of Human IL-1 Beta is greater than 95% as determined by SEC-HPLC.
	Human IL-1 Beta on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.