Human IL-11 Protein (IL1-HM111)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-11, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Pro22-Leu199

Catalog: IL1-HM111

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Product Description	Recombinant Human IL-11 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Pro22-Leu199.
Product Background	Abstract Interleukin-11 (IL-11) is a pleiotropic cytokine that belongs to gp130 family. IL-11 and its receptor, IL-11Ra, are expressed in human cancers,human cancer cells expressed a functional IL-11Ra subunit, which triggered signal transduction either by exogenous recombinant human IL-11 or by autocrine production of IL-11 in cells cultured under hypoxic conditions.
Product Category	Recombinant Protein

Target	IL-11
Synonyms	Interleukin-11;IL11;Adipogenesis inhibitory factor;AGIF;INN: Oprelvekin;Oprelvekin;IL-11Oprelvekin;interleukin 11;interleukin-11
Gene/Protein ID	P20809-1
Species	Human
Exact Sequence	Pro22-Leu199
Molecular Weight	The protein has a predicted MW of 20.24 kDa. Due to glycosylation, the protein migrates to 21-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-11, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test). The affinity constant of 1.86 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-11R alpha, His Tag immobilized on CM5 Chip can bind Human IL-11, His Tag with an affinity constant of 1.86 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-11, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-11 R alpha, hFc Tag with the EC50 of 26.2ng/ml determined by ELISA (QC Test).
	The purity of Human IL-11 is greater than 95% as determined by SEC-HPLC.
	Human IL-11 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.