Human IL-15 Protein (IL5-HE015)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. The affinity constant of 5.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Asn49-Ser162

Catalog: IL5-HE015

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Product Description	Recombinant Human IL-15 Protein is expressed from E.coli without tag.It contains Asn49-Ser162.
Product Background	Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases.IL-15 is a cytokine that stimulates the proliferation of T-lymphocytes. Stimulation by IL15 requires interaction of IL15 with components of the IL2 receptor, including IL2RB and probably IL2RG but not IL2RA.
Product Category	Recombinant Protein

Target	IL-15
Synonyms	L15; IL-15; IL-15MGC9721; Interleukin-15
Gene/Protein ID	P40933
Species	Human
Exact Sequence	Asn49-Ser162
Molecular Weight	The protein has a predicted MW of 12.8 kDa same as Tris-Bis PAGE result.
Activity	Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA. The affinity constant of 5.9nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-15, No Tag with an affinity constant of 5.9nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-15RA, His Tag with the EC50 of 22.1ng/ml determined by ELISA.
	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50-for this effect is 1-2 ng/mL. The specific activity of recombinant human IL-15 is > 5 x 10^7-units/mg, which is calibrated against the human IL-15 reference standard (NIBSC code: 95/554) (QC Test).
	Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Bioactivity of three different batches of Human IL-15 shows very high batch-to-batch consistency.
	The purity of Human IL-15 is greater than 95% as determined by SEC-HPLC.
	Human IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.