Human IL-17RB Protein (IL1-HM1RB)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Arg18-Per292

Catalog: IL1-HM1RB

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Product Description	Recombinant Human IL-17RB Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg18-Per292.
Product Background	Among inflammatory mediators, a growing body of evidence emphasizes the contribution of the interleukin 17 (IL-17) cytokine family in malignant diseases. Besides IL-17A, the prototypic member of the IL-17 family, several experimental findings strongly support the role of the IL-17B/IL-17 receptor B (IL-17RB) pathway in tumorigenesis and resistance to anticancer therapies. In mouse models, IL-17B signaling through IL-17RB directly promotes cancer cell survival, proliferation, and migration, and induces resistance to conventional chemotherapeutic agents.
Product Category	Recombinant Protein

Target	IL-17RB
Synonyms	IL-17 receptor B; IL-17RB; IL-17ER; Evi27; IL-17 RH1; IL-17ER; IL17RB
Gene/Protein ID	Q9NRM6-1
Species	Human
Exact Sequence	Arg18-Per292
Molecular Weight	The protein has a predicted MW of 31.4 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test). The affinity constant of 0.81 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-17RB, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-17RB Antibody, hFc Tag with the EC50 of 5.4ng/ml determined by ELISA (QC Test).
	Immobilized Human IL-17RB, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-25, His Tag with the EC50 of 58.5ng/ml determined by ELISA.
	The purity of Human IL-17RB is greater than 95% as determined by SEC-HPLC.
	Human IL-17RB on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.