Human IL-18 Protein (IL1-HE018)
Catalog: IL1-HE018
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Product Description | Recombinant Human IL-18 Protein is expressed from E.coli without tag.It contains Tyr37-Asp193. |
Product Background |
Interleukin (IL)-18 was originally discovered as a factor that enhanced IFN-γ production from anti-CD3-stimulated Th1 cells, especially in the presence of IL-12. Upon stimulation with Ag plus IL-12, naïve T cells develop into IL-18 receptor (IL-18R) expressing Th1 cells, which increase IFN-γ production in response to IL-18 stimulation. |
Product Category |
Recombinant Protein |
Target |
IL-18 |
Synonyms | IL-18; IL18; IGIF; IL-1F4; IL-1 gamma; IL1F4; IL-1g; Interleukin 1 |
Gene/Protein ID |
Q14116 |
Species |
Human |
Exact Sequence |
Tyr37-Asp193 |
Molecular Weight | The protein has a predicted MW of 18.2 kDa same as Tris-Bis PAGE result. |
Activity |
Immobilized Human IL-18 at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-18BP, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 2.38 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
No Tag |
Express System |
E.coli |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 20mM PB, 250mM NaCl, 50mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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