Human IL-1R1 Protein (IL1-HM1R1)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-1R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Leu18-Thr332

Catalog: IL1-HM1R1

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Product Description	Recombinant Human IL-1R1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu18-Thr332.
Product Background	Interleukin 1 (IL-1) has long been known for its pleiotropic effects on inflammation that plays a complex, and sometimes contrasting, role in different stages of cancer development.IL-1R1 is the main receptor for both ligands and is expressed by various cell types, including innate and adaptive immune cell types, epithelial cells, endothelial cells, adipocytes, chondrocytes, fibroblasts, etc. IL-1 and IL-1R1 receptor interaction leads to a set of common signaling pathways, mainly the NF-kB and MAP kinase pathways, as a result of complex positive and negative regulations.
Product Category	Recombinant Protein

Target	IL-1R1
Synonyms	IL-1R-1; IL-1RT1; IL-1R-alpha; CD121a; IL-1 RI; IL1R; IL1RI; IL-1RI; p80; D2S1473; IL1RA
Gene/Protein ID	NP_000868.1
Species	Human
Exact Sequence	Leu18-Thr332
Molecular Weight	The protein has a predicted MW of 37 kDa. Due to glycosylation, the protein migrates to 55-68 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-1R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test). The affinity constant of 0.51 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-1R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-1 alpha, No tag with an affinity constant of 0.51 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-1R1, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta, His Tag with the EC50 of 0.13ug/ml determined by ELISA (QC Test).
	The purity of Human IL-1R1 is greater than 95% as determined by SEC-HPLC.
	Human IL-1R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.