Human IL-2 Protein (IL2-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Ala21-Thr153

Catalog: IL2-HE001

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Product Description	Recombinant Human IL-2 Protein is expressed from E.coli without tag.It contains Ala21-Thr153.
Product Background	Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Product Category	Recombinant Protein

Target	IL-2
Synonyms	IL2; T-cell growth factor; TCGF; Aldesleukin
Gene/Protein ID	P60568
Species	Human
Exact Sequence	Ala21-Thr153
Molecular Weight	The protein has a predicted 15.5 kDa same as Tris-Bis PAGE result.
Activity	The affinity constant of 16.82 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 100mM Acetic Acid. Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 100mM Acetic Acid.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-2, No Tag with an affinity constant of 16.82 nM as determined in SPR assay (Biacore T200).
	Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
	Human IL-2 R beta, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human IL-2, No Tag with an affinity constant of 0.25uM as determined in SPR assay (Biacore T200).
	Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 0.5-2 ng/mL (QC Test).
	The purity of Human IL-2 is greater than 95% as determined by SEC-HPLC.
	Human IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.