Human IL-2 R alpha/CD25 Protein (CD5-HM425)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-2 R alpha, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Glu22-Cys213

Catalog: CD5-HM425

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Product Description	Recombinant Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu22-Cys213.
Product Background	Human IL-2 receptor alpha (IL-2 R alpha), also known as Tac antigen and as CD25, was initially identified as a 55 kDa membrane glycoprotein that is capable of binding IL‑2.eceptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.
Product Category	Recombinant Protein

Target	IL-2 R alpha/CD25
Synonyms	CD25; IDDM10; IL-2 R alpha; IL2R; IL2RA; p55; TCGFR
Gene/Protein ID	P01589
Species	Human
Exact Sequence	Glu22-Cys213
Molecular Weight	The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-2 R alpha, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test). The affinity constant of 15.2nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-2 R alpha, His Tag captured on CM5 Chip via anti-His antibody can bind Human IL-2, No Tag with an affinity constant of 15.2nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-2 R alpha, His Tag at 0.2ug/ml (100ul/well) on the plate. Dose response curve for Anti-IL-2 R alpha Antibody, hFc Tag with the EC50 of 9.6ng/ml determined by ELISA (QC Test).
	The purity of Human IL-2 R alpha is greater than 95% as determined by SEC-HPLC.
	Human IL-2 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.