Human IL-2 R beta&IL-2 R gamma Protein (ILR-HM2BG)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)

Catalog: ILR-HM2BG

$315.00
Size:

Order now get it on

Bulk Quote | Test Sample
Product Description Recombinant Human IL-2 R beta&IL-2 R gamma Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala27-Thr240 (IL-2 R beta)&Leu23-Ala262 (IL-2 R gamma).

Product Background

 Wilms' tumor 1 (WT1) is a transcription factor which plays a major role in cell proliferation, differentiation, survival, and apoptosis. WT1 was first identified as a tumor suppressor gene in Wilms' tumor. The expression of survival signaling genes, IL-2, IL-2RB, and IL-2RG, was also suppressed after WT1-siRNA treatment. In addition, the WT1 silencing also inhibited the S phase of the cell cycle and induced cell death.Moreover, transcriptional modulation of IL-2, IL-2RB, and IL2-2RG expression by WT1 was likely involved in this phenotypic change.

Product Category

 Recombinant Protein

Target

 IL-2 R beta&IL-2 R gamma
Synonyms IL-2RB&IL-2RG; IL-2RG; IL-2R subunit gamma; IL-2R subunit beta; IL-2RB; IL15RB; IL-2 R beta&IL-2 R gamma

Gene/Protein ID

 P14784(IL-2 R beta)&P31785-1(IL-2 R gamma)

Species

 Human

Exact Sequence

 Ala27-Thr240(IL-2 R beta) & Leu23-Ala262(IL-2 R gamma)
Molecular Weight The protein has a predicted MW of 51.3 kDa (IL-2 R beta)&54.6 kDa (IL-2 R gamma). Due to glycosylation, the protein migrates to 65-70 kDa&80-100 kDa based on Tris-Bis PAGE result.

Activity

 Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.114 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

 C-hFc

Express System

 HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-2, No Tag with an affinity constant of 0.114 nM as determined in SPR assay (Biacore T200).
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).
The purity of Human IL-2 R beta&IL-2 R gamma is greater than 95% as determined by SEC-HPLC.
Human IL-2 R beta&IL-2 R gamma on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in

View more associated products