Human JAM-A Protein (JAM-HM10A)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human JAM-A, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser28-Val238.

Catalog: JAM-HM10A

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Product Description	Recombinant Human JAM-A Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser28-Val238.
Product Background	junctional adhesion molecule A (JAM-A), a cell adhesion molecule, is highly elevated in human GBM cancer stem cells and predicts poor patient prognosis. While JAM-A is also highly expressed in other cells in the tumor microenvironment, specifically microglia and macrophages,JAM-A functions to suppress pathogenic microglial activation in the female tumor microenvironment, highlighting an emerging role for sex differences in the GBM microenvironment and suggesting that sex differences extend beyond previously reported tumor cell-intrinsic differences.
Product Category	Recombinant Protein

Target	JAM-A
Synonyms	JAM-A; JAM-1; CD321; JAM; JCAM; JCAM1; KAT; PAM-1; F11 receptor; F11R; PAM-1KAT
Gene/Protein ID	Q9Y624-1
Species	Human
Exact Sequence	Ser28-Val238.
Molecular Weight	The protein has a predicted MW of 24 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human JAM-A, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human JAM-A, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-JAM-A Antibody, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA.
	Human JAM-A on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.