Human LIF R/CD118 Protein (LIF-HM10R)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln45-Ser833

Catalog: LIF-HM10R

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human LIF R/CD118 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln45-Ser833.
Product Background	The leukemia inhibitory factor receptor (LIF-R) subunit is a component of cell-surface receptor complexes for the multifunctional cytokines, LIF, cardiotrophin-1, ciliary neurotrophic factor, and human oncostatin M. The structure of the human LIF-R gene is similar to that of the mouse gene. The transmembrane receptor is encoded by 19 exons.
Product Category	Recombinant Protein

Target	LIF R/CD118
Synonyms	FLJ99923; SJS2; STWS; SWS; LIFR; CD118; FLJ98106
Gene/Protein ID	P42702-1
Species	Human
Exact Sequence	Gln45-Ser833
Molecular Weight	The protein has a predicted MW of 90.5 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test). The affinity constant of 0.662 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human LIF R, His Tag immobilized on CM5 Chip can bind Human Oncostatin M, His Tag with an affinity constant of 0.662 uM as determined in SPR assay (Biacore T200).
	Immobilized Human LIF at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human LIF R, His Tag with the EC50 of 0.30ug/ml determined by ELISA (QC Test).
	The purity of Human LIF R/CD118 is greater than 95% as determined by SEC-HPLC.
	Human LIF R/CD118 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.