Human LIGHT/TNFSF14 Protein (LGT-HM131)

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Asp74-Val240

Catalog: LGT-HM131

Order now get it on

Bulk Quote | Test Sample

Product Description	Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Asp74-Val240.
Product Background	LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Product Category	Recombinant Protein

Target	LIGHT/TNFSF14
Synonyms	CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Gene/Protein ID	O43557-1
Species	Human
Exact Sequence	Asp74-Val240
Molecular Weight	The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LIGHT, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3ng/ml determined by ELISA (QC Test).
	Immobilized Human LIGHT, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 22.9ng/ml determined by ELISA.
	Human LIGHT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.