Human LIGHT/TNFSF14 Trimer Protein (LGT-HM132)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 90% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LIGHT Trimer, His tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 86.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ser89-Val240

Catalog: LGT-HM132

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Product Description	Recombinant Human LIGHT/TNFSF14 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ser89-Val240 Trimer.
Product Background	LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3.This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Product Category	Recombinant Protein

Target	LIGHT/TNFSF14 Trimer
Synonyms	CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Gene/Protein ID	O43557-1
Species	Human
Exact Sequence	Ser89-Val240
Molecular Weight	The protein has a predicted MW of 52.4 kDa. Due to glycosylation, the protein migrates to 53-140 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.
Activity	Immobilized Human LIGHT Trimer, His tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 86.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 90% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LIGHT Trimer, His tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 86.3ng/ml determined by ELISA.
	The purity of Human LIGHT Trimer is greater than 95% as determined by SEC-HPLC.
	Human LIGHT Trimer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 90%.