Human LILRB1/CD85j/ILT2 Domain1&2-Protein (LIL-HM11D)
Catalog: LIL-HM11D
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| Product Description | Recombinant Human LILRB1/CD85j/IL-T2 Domain1&2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly24-Gly221. |
Product Background |
LILRB1, also known as CD85j and IL-T2, is a 110 kDa transmembrane glycoprotein in the LILR immunoregulatory protein family. Mature human LILRB1 consists of a 438 amino acid (aa) extracellular domain (ECD) with 4 tandem Ig-like domains, a 21 aa transmembrane segment, and a 168 aa cytoplasmic domain with 4 inhibitory ITIM motifs.LILRB1 is a receptor for class I MHC antigens. |
Product Category |
Recombinant Protein |
Target |
LILRB1/CD85j/ILT2 Domain1&2 |
| Synonyms | ILT2; ILT-2; ILT2FLJ37515; LILRB1; LIR1; MIR7; CD85J; XXbac-BCX85G21.4 |
Gene/Protein ID |
Q8NHL6-1 |
Species |
Human |
Exact Sequence |
Gly24-Gly221 |
| Molecular Weight | The protein has a predicted MW of 23.1 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human LILRB1 Domain1&2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). The affinity constant of 1.9uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human HLA-G Tetramer captured on CM5 Chip can bind Human LILRB1 Domain1&2, His Tag with an affinity constant of 1.9uM as determined in SPR assay (Biacore T200). |
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Immobilized Human LILRB1 Domain1&2, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LILRB1 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA (QC Test). |
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Serial dilutions of Anti-LILRB1 Antibody were added into Biotinylated Human HLA-G Complex Tetramer, His Tag : Human LILRB1 Domain1&2, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 34.6ng/ml. |
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The purity of Human LILRB1 Domain1&2 is greater than 95% as determined by SEC-HPLC. |
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Human LILRB1 Domain1&2 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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