Human MASP2 Protein (MSP-HE102)

Express System: E.coli
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human MASP2, His Tag at 0.2ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gly288-Phe686

Catalog: MSP-HE102

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Product Description	Recombinant Human MASP2 Protein is expressed from E.coli with His tag at the C-Terminus.It contains Gly288-Phe686.
Product Background	The pathogenesis of severe acute respiratory disease syndrome (SARS) is not fully understood. One case-control study has reported an association between susceptibility to SARS and mannan-binding lectin (MBL) in China. As the downstream protein of MBL, variants of the MBL-associated serine protease-2 (MASP2) gene may be associated with SARS coronavirus (SARS-CoV) infection in the same population.
Product Category	Recombinant Protein

Target	MASP2
Synonyms	MAP19; MASP-2; MASP1P1; sMap
Gene/Protein ID	O00187-1
Species	Human
Exact Sequence	Gly288-Phe686
Molecular Weight	The protein has a predicted MW of 46.9 kDa. Due to autocatalytic cleavage, the protein migrates to 18 kDa&25-30 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MASP2, His Tag at 0.2ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test). The affinity constant of 3.08 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 200mM NaCl (pH 9.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-MASP2 Antibody captured on CM5 Chip via Protein A can bind Human MASP2, His Tag with an affinity constant of 3.08 nM as determined in SPR assay (Biacore T200).
	Immobilized Human MASP2, His Tag at 0.2ug/ml (100ul/Well). Dose response curve for Anti-MASP2 Antibody, hFc Tag with the EC50 of 13.8ng/ml determined by ELISA (QC Test).
	Human MASP2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.