Human/Murine/Rat BDNF-Protein (BDF-HE001)

Express System: E.coli
Product Tag: No Tag
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: His129-Arg247

Catalog: BDF-HE001

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Product Description	Recombinant Human/Murine/Rat BDNF-Protein is expressed from E.coli without tagIt contains His129-Arg247.
Product Background	The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.
Product Category	Recombinant Protein

Target	BDNF
Gene/Protein ID	P23560-1
Species	Human/Murine/Rat
Exact Sequence	His129-Arg247
Molecular Weight	The protein has a predicted MW of 13.51 kDa. The protein migrates to 15-19 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag	No Tag
Express System	E.coli
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human/Murine/Rat BDNF, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.17ug/ml determined by ELISA.
	Human/Murine/Rat BDNF on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.