Human Nectin-4 Protein IgV Domain (NEC-HM414)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Nectin-4 IgV Domain, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Gly32-Leu146

Catalog: NEC-HM414

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Product Description	Recombinant Human Nectin-4 Protein IgV Domain is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Leu146.
Product Background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category	Recombinant Protein

Target	Nectin-4 IgV Domain
Synonyms	EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Gene/Protein ID	Q96NY8-1
Species	Human
Exact Sequence	Gly32-Leu146
Molecular Weight	The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Nectin-4 IgV Domain, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Nectin-4 IgV Domain, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.
	The purity of Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.
	Human Nectin-4 IgV Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.