Human Neogenin Protein (NEO-HM201)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala34-Leu1105

Catalog: NEO-HM201

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Product Description	Recombinant Human Neogenin Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala34-Leu1105.
Product Background	Neogenin is a multifunctional transmembrane receptor belonging to the immunoglobulin superfamily. It displays identical secondary structure to deleted in colorectal cancer (DCC), a netrin receptor that is involved in axon guidance and cell survival.
Product Category	Recombinant Protein

Target	Neogenin
Gene/Protein ID	Q92859-1
Species	Human
Exact Sequence	Ala34-Leu1105
Molecular Weight	The protein has a predicted MW of 144.37 kDa. Due to glycosylation, the protein migrates to 150-180 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human RGMa, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human Neogenin, hFc Tag with the EC50 of 0.17ug/ml determined by ELISA.
	The purity of Human Neogenin is greater than 95% as determined by SEC-HPLC.
	Human Neogenin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.