Human Neuropilin-1 Protein (NRP-HM101)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Phe22-Lys644

Catalog: NRP-HM101

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Product Description	Recombinant Human Neuropilin-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Phe22-Lys644.
Product Background	Neuropilin 1 (NRP1) is a transmembrane glycoprotein that acts as a co-receptor for a number of extracellular ligands including class III/IV semaphorins, certain isoforms of vascular endothelial growth factor and transforming growth factor beta.
Product Category	Recombinant Protein

Target	Neuropilin-1
Gene/Protein ID	NP_001019799.1
Species	Human
Exact Sequence	Phe22-Lys644
Molecular Weight	The protein has a predicted MW of 70.97 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human VEGF165, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Neuropilin-1, His Tag with the EC50 of 0.57ug/ml determined by ELISA.
	The purity of Human Neuropilin-1 is greater than 95% as determined by SEC-HPLC.
	Human Neuropilin-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.