Human NKG2D/CD314 Protein (NKG-HM42D)

Express System: HEK293
Product Tag: N-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE
Activity: Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Phe78-Val216

Catalog: NKG-HM42D

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Product Description	Recombinant Human NKG2D/CD314 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Phe78-Val216.
Product Background	NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Product Category	Recombinant Protein

Target	NKG2D/CD314
Synonyms	CD314; D12S2489E; KLR; NKG2-D; NKG2D
Gene/Protein ID	P26718
Species	Human
Exact Sequence	Phe78-Val216
Molecular Weight	The protein has a predicted MW of 19.0 kDa. Due to glycosylation, the protein migrates to 36-38 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test). The affinity constant of 95.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	N-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human MICB, hFc Tag captured on CM5 Chip via Protein A can bind Human NKG2D, His Tag with an affinity constant of 95.15 nM as determined in SPR assay (Biacore T200).
	Immobilized Human NKG2D, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-NKG2D Antibody, hFc Tag with the EC50 of 6.6ng/ml determined by ELISA (QC Test).
	Immobilized Human NKG2D, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human MICA, hFc Tag with the EC50 of 33.4ng/ml determined by ELISA.
	Immobilized Human NKG2D, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human ULBP-6, hFc Tag with the EC50 of 12.9ng/ml determined by ELISA.
	Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.