Human NRAS-Protein (NRS-HE101)

  • Express System: E.coli
  • Product Tag: N-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Exact Sequence: Met1-Cys186

Catalog: NRS-HE101

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Product Description Recombinant Human NRAS-Protein is expressed from E.coli with His tag at the N-Terminus.It contains Met1-Cys186.

Product Background

 NRas is a key mediator of the mitogenic pathway in normal cells and in cancer cells. Its dynamics and nanoscale organization at the plasma membrane (PM) facilitate its signaling. NRas activity is mediated and regulated by its dynamic interactions with functional (Grb2, BRAF, NF1, and GPI-nucleated) clusters at the PM.

Product Category

 Recombinant Protein

Target

 NRAS

Gene/Protein ID

 P01111

Species

 Human

Exact Sequence

 Met1-Cys186
Molecular Weight The protein has a predicted MW of 22.29 kDa same as Tris-Bis PAGE result.

Product Tag

 N-His

Express System

 E.coli
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The purity of Human NRAS-is greater than 90% as determined by SEC-HPLC.
Human NRAS on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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