Human OSMR&GP130 Protein (OGC-HM10R)

Express System: HEK293
Product Tag: C-His, C-Flag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu28-Met740(OSMR) & Glu23-Glu619(GP130)

Catalog: OGC-HM10R

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Product Description	Recombinant Human OSMR&GP130 Protein is expressed from HEK293 with His tag and Flag tag at the C-Terminus.It contains Glu28-Met740(OSMR)&Glu23-Glu619(GP130).
Product Background	OSMR&gp130 is a type II receptor for OSM in humans. OSM can alternatively bind to a heterodimer of gp130 and LIF-R or of gp130 and OSMR (OSM receptor). On the basis of in vitro studies, the OSM ligand recruits a broader array of cell signaling pathways than most gp130 cytokines. OSMR and gp130 chains are widely expressed in connective tissue cells and tumor cells.
Product Category	Recombinant Protein

Target	OSMR&GP130
Gene/Protein ID	Q99650-1(OSMR)&P40189-1(GP130)
Species	Human
Exact Sequence	Glu28-Met740(OSMR) & Glu23-Glu619(GP130)
Molecular Weight	The protein has a predicted MW of 82.2 kDa(OSMR) and 68.83 kDa(GP130). Due to glycosylation, the protein migrates to 100-140 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His, C-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human OSMR&GP130, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-OSMR Antibody, hFc Tag with the EC50 of 2.8ng/ml determined by ELISA.
	The purity of Human OSMR&GP130 is greater than 95% as determined by SEC-HPLC.
	Human OSMR&GP130 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.