Human PAI-1 Protein (PAI-HM101)
Catalog: PAI-HM101
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| Product Description | Recombinant Human PAI-1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val24-Pro402. |
Product Background |
PAI-1 (plasminogen activator inhibitor-1) is a member of plasminogen cascade with an inhibitory role in plasmin activation. PAI-1 is an important regulator of the fibrinolytic process and levels of this antifibrinolytic protein are elevated in diabetes and insulin resistant states. |
Product Category |
Recombinant Protein |
Target |
PAI-1 |
Gene/Protein ID |
P05121-1 |
Species |
Human |
Exact Sequence |
Val24-Pro402 |
| Molecular Weight | The protein has a predicted MW of 43.86 kDa. Due to glycosylation, the protein migrates to 45-55 kDa based on Tris-Bis PAGE result. |
Product Tag |
C-His |
Express System |
HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22 um filtered solution in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 50mM NaAC, 0.1M NaCl, 100mM L-arginine (pH 5.5).. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 35 nM. |
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The purity of Human PAI-1 is greater than 95% as determined by SEC-HPLC. |
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Human PAI-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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