Human PCSK9 Protein (PCS-HM190)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human PCSK9, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln31-Gln692

Catalog: PCS-HM190

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Product Description	Recombinant Human PCSK9 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln31-Gln692.
Product Background	Proprotein convertase subtilisin/kexin type 9 (PCSK9) is an enzyme encoded by the PCSK9 gene in humans on chromosome 1.The first two PCSK9 inhibitors, alirocumab and evolocumab, were approved as once every two week injections, by the U.S. Food and Drug Administration in 2015 for lowering LDL-particle concentrations when statins and other drugs were not sufficiently effective or poorly tolerated.
Product Category	Recombinant Protein

Target	PCSK9
Synonyms	PC9; PCSK9; FH3; HCHOLA3; LDLCQ1; NARC-1
Gene/Protein ID	Q8NBP7-1
Species	Human
Exact Sequence	Gln31-Gln692
Molecular Weight	Due to autocatalytic cleavage, the protein release the pro-form (59 kDa) and mature form (14 kDa). Due to glycosylation, the protein migrates to 65-68 kDa (pro-form) and 15kDa (mature form) based on Tris-Bis PAGE result.
Activity	Immobilized Human PCSK9, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test). The affinity constant of 0.35 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Liquid
Shipping	Shipped with dry ice.
Formulation	Supplied as 0.22um filtered solution in 20mM PB, 300mM NaCl, 10% Glycerol (pH 7.4).
Stability And Storage	Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human LDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.35 nM as determined in SPR assay (Biacore T200).
	Immobilized Human PCSK9, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-PCSK9 antibody, hFc Tag with the EC50 of 0.16ug/ml determined by ELISA (QC Test).
	The purity of Human PCSK9 is greater than 95% as determined by SEC-HPLC.
	Human PCSK9 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.